Science and Nature

Prenatal maternal infection promotes tissue-particular immunity and irritation in offspring

Mother’s IL-6 rewires puny one’s gut immunity

Most infections that occur all via pregnancy are gentle and transient. Then all over again, whether or no longer such pathogen encounters can shape the lengthy-term trajectory of the offspring’s immune system stays unclear. Lim et al. contaminated pregnant mice with the frequent meals-borne pathogen Yersinia pseudotuberculosis (YopM) (gape the Perspective by Amir and Zeng). Despite the truth that the infection used to be maternally restricted and short-lived, the offspring harbored higher numbers of intestinal T helper 17 cells into maturity. Interleukin-6 (IL-6) mediated this tissue-restricted live by performing on the fetal intestinal epithelium all via pattern. Despite the truth that offspring from moms contaminated with YopM or injected with IL-6 showed enhanced resistance to oral infection with Salmonella Typhimurium, they also exhibited better susceptibility in direction of enteric inflammatory disease.

Science, abf3002, this convey p. eabf3002; gape also abl3631, p. 967

Structured Abstract

INTRODUCTION

One traditional property of the immune system is its ability to contain memory of old encounters, ensuing in enhanced responsiveness to subsequent challenges. This phenomenon contains no longer most productive the adaptive immune system nonetheless also innate cells and tissue stem cells. The notion that of host imprinting by infection main to altered responses to subsequent convey is of particular curiosity in the context of pregnancy, which represents a common developmental window for the immune system.

RATIONALE

Most infections encountered by mammals, collectively with those skilled all via pregnancy, are gentle and transient. How these infections shape offspring tissue immunity and tissue predisposition to inflammatory concerns in the extinguish stays to be addressed.

RESULTS

Infection of timed-pregnant dams (day 10.5) with an attenuated stress of the meals-borne pathogen Yersinia pseudotuberculosis (yopM) used to be transient and maternally restricted. Grownup offspring of beforehand contaminated dams harbored a better quantity of T helper 17 (TH17) cells nonetheless no other cell subsets in the small and orderly intestinal lamina propria. No changes had been seen at other barrier tissues. Transfer of transgenic T cells particular for a commensal antigen published that maternal infection affected the offspring intestinal milieu in a vogue that enhanced TH17 cell reactivity in direction of the microbiota. Dreadful-fostering experiments demonstrated that the elevated TH17 cells attributable to maternal infection used to be imprinted in utero. Among assorted inflammatory mediators, the cytokine interleukin-6 (IL-6) used to be seriously elevated in the serum of dams contaminated with yopM. Injection of IL-6 on my own to pregnant dams seriously elevated TH17 cell numbers within the heart of offspring. Conversely, blockade of IL-6 in contaminated dams averted this enhance in offspring. Furthermore, injection of IL-6 to germ-free pregnant dams and conventionalization of their offspring after weaning published that prenatal institution and postnatal maintenance of IL-6–mediated tissue imprinting are neutral of the maternal microbiota nonetheless allow the offspring to mount enhanced TH17 cell responses to postnatal microbiota exposure.

IL6RA used to be expressed in all fetal intestinal epithelial cells, and particular deletion of IL6RA from epithelial cells seriously diminished Th17 responses within the gut of offspring from IL-6–uncovered dams. Using complementary approaches, collectively with assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) and single-cell RNA sequencing, we chanced on that elevated IL-6 all via pregnancy had instantaneous effects (on fetal cells) and lengthy-term effects (in the adult offspring) on epithelial stem cell chromatin accessibility and downstream epithelial cell feature, namely elevated expression of genes encoding for antigen presentation machinery and antimicrobial peptides.

Altered epithelial activation jam instructed the chance that this phenomenon will seemingly be associated to enhanced antimicrobial defense. To address this, we used an acute model of oral infection with Salmonella Typhimurium. Offspring from dams beforehand contaminated with yopM or injected with IL-6 all via pregnancy developed enhanced resistance to Salmonella oral infection and dissemination. No variations had been seen in controlling cutaneous Candida albicans infection, extra supporting the notion that maternal imprinting of offspring is proscribed to the gut compartment. Then all over again, enhanced exposure to IL-6 all via pregnancy used to be associated to enhanced gut pathology in the context of naïve T cell transfer and dextran sulfate sodium–mediated colitis.

CONCLUSION

Our work proposes that a transient, gentle infection encountered all via prenatal pattern can impose lasting alterations to gut epithelial stem cells, ensuing in an altered threshold of activation and enhanced resistance to gut infection. The affect of maternal infection used to be tissue particular and predominantly mediated by a single cytokine, IL-6, performing on epithelial stem cells all via fetal pattern. Despite the truth that this phenomenon shall be coopted by the fetus to contain optimum immune fitness, altered offspring immunity imposed by maternal infection comes on the pricetag of enhanced susceptibility to mucosal irritation.

Maternal infection promotes offspring intestine-specific immunity and inflammation.

The direct response of fetal intestinal epithelial cells to IL-6 during maternal infection confers an enduring epigenetic memory to adult intestinal epithelial stem cells. As such, offspring epithelial cells exhibit enhanced reactivity toward the microbiota and heightened ability to control oral infection. However, these responses come at the cost of greater predisposition to gut inflammation.

” data-hide-link-title=”0″ data-icon-position=”” href=”http://science.sciencemag.org/content/sci/373/6558/eabf3002/F1.large.jpg?width=800&height=600&carousel=1″ rel=”gallery-fragment-images-192185586″ title=”Maternal infection promotes offspring intestine-specific immunity and inflammation. The direct response of fetal intestinal epithelial cells to IL-6 during maternal infection confers an enduring epigenetic memory to adult intestinal epithelial stem cells. As such, offspring epithelial cells exhibit enhanced reactivity toward the microbiota and heightened ability to control oral infection. However, these responses come at the cost of greater predisposition to gut inflammation.”>

Maternal infection promotes offspring intestine-particular immunity and irritation.

The advise response of fetal intestinal epithelial cells to IL-6 all via maternal infection confers a lengthy-lasting epigenetic memory to adult intestinal epithelial stem cells. As such, offspring epithelial cells verbalize enhanced reactivity in direction of the microbiota and heightened ability to manipulate oral infection. Then all over again, these responses reach on the pricetag of higher predisposition to gut irritation.

Abstract

The immune system has evolved in the face of microbial exposure. How maternal infection skilled at certain developmental levels shapes the offspring immune system stays poorly understood. Here, we verbalize that all via pregnancy, maternally restricted infection can possess permanent and tissue-particular impacts on offspring immunity. Mechanistically, maternal interleukin-6 produced in accordance with infection can without lengthen impose epigenetic changes on fetal intestinal epithelial stem cells, main to lengthy-lasting impacts on intestinal immune homeostasis. Which potential that, offspring of beforehand contaminated dams contain enhanced protective immunity to gut infection and elevated irritation in the context of colitis. Thus, maternal infection shall be coopted by the fetus to promote lengthy-term, tissue-particular fitness, a phenomenon that will reach on the pricetag of predisposition to inflammatory concerns.

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